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Catalog Number | orb1174023 |
---|---|
Category | Antibodies |
Description | Anti-CD155 antibody(DM146), Rabbit mAb |
Target | CD155 |
Clonality | Monoclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Liquid |
UniProt ID | P15151 |
Tested applications | ELISA, FC |
Dilution range | ELISA 1/5000-10000|Flow Cyt 1/100 |
Application notes | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Antibody Type | Recombinant Antibody |
Clone Number | DM146 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | PVR; FLJ25946; PVS; CD155; TAGE4; HVED; NECL5 |
Note | For research use only |
ELISA plate pre-coated by 1 μg/ml (100 μL/well) Human CD155 protein, mFc tagged protein orb689388 can bind Rabbit anti-CD155 monoclonal antibody (clone: DM146) in a linear range of 5-100 ng/ml.
CD155 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CD155 on Expi293 cells transfected with human CD155 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).
Flow cytometry analysis of antigen binding of rabbit anti-human CD155 mAb (orb1174023). (A) orb1174023 does not bind to CHO-S cells that do not express CD155. (B) A clear peak shift of orb1174023 was seen compared to the control when incubated with CD155-expressing Daudi cells, indicating strong binding of orb1174023 to CD155. Antibodies were incubated at 5 μg/ml.
ELISA, FC | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |