Anti-CD154 [YMF323.6]

• Catalog Number: orb613939
• Category: Antibodies
• Description: Rat monoclonal antibody to CD154
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: YMF323.6
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: IgG2a
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD154
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD40L; CD40 Ligand; HIGM1; IGM; IMD3; T-BAM; TNFSF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-CD154 antibody YMF323.6. Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YMF323.6 (orb613938) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613938, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.