Anti-CD154 [YMF323.6]

SKU: orb613939

Description

Rat monoclonal antibody to CD154

Images & Validation

• Tested Applications: FC, WB
• Reactivity: Human

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: YMF323.6
• Target: CD154
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD40L; CD40 Ligand; HIGM1; IGM; IMD3; T-BAM; TNFSF5;T RAP; gp39; hCD40L

Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-CD154 antibody YMF323.6. Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YMF323.6 (orb613938) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613938, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD154