Anti-CD153 [RM153]

• Catalog Number: orb758821
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD153
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: RM153
• Tested applications: Blocking, FA, FC
• Reactivity: Mouse
• Isotype: Rat IgG
• Immunogen: This antibody was raised by immunising Sprague Dawley rats with the CHO cells stably transfected with murine CD153 and fusing the splenocytes with P3U1 myeloma cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD153
• UniProt ID: P32972
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD30-L; CD30 ligand; Tumor necrosis factor ligand
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed RAW264.7 cells with anti-CD153 antibody RM153. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of RG9-35 (orb758821) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758821, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.