CD142 Biosimilar Antibody

• Catalog Number: orb1291207
• Category: Antibodies
• Description: Anti-F3(tisotumab biosimilar) mAb
• Target: CD142
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Powder
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P13726
• Tested applications: ELISA, FC
• Dilution range: ELISA 1:5000-10000, Flow Cyt 1:100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Biosimilar Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Tissue factor, TF, F3, Thromboplastin, Coagulation
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD142 Protein, hFc Tag (orb1290926) can bind Anti-CD142 Neutralizing antibody (orb1291207) in a linear range of 2.93–187.50 ng/ml. In order to specifically detect orb1291207, mouse anti-human Fab-specific antibody was used as detection antibody.

Flow cytometry analysis of antigen binding of anti-human CD142 mAb (orb1291207). (A) orb1291207 does not bind to CHO-S cells that do not express CD142. (B) A clear peak shift of orb1291207 was seen compared to the control when incubated with CD142-expressing Hela cells, indicating strong binding of orb1291207 to CD142. Antibodies were incubated at 5 μg/mL.