CD142 Antibody

SKU: orb1474472

Description

The CD142 Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against A synthetic peptide of human Tissue Factor .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Tissue Factor
• Purification: The antibody was purified by immunogen affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Disclaimer: For research use only

Alternative Names

Tissue factor; TF; Coagulation factor III; Thromboplastin; CD142

Western blot analysis of CD142 expression in Hela (A), A549 (B), MCF7 (C), U251 (D), HL60 (E), mouse brain (F), rat brain (G) whole cell lysates. (Predicted band size: 33 kD; Observed band size: 45 kD)

Immunohistochemical analysis of CD142 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.88). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P13726, P42533, P20352

NCBI Gene ID

14066, 2152