CD137 Rat Monoclonal Antibody

SKU: orb1293360

Description

The CD137 Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Mouse CD137 human Fc fusion protein .Purification: The antibody was purified by affinity chromatography.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF
• Dilution Range: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
• Reactivity: Mouse
• Application Notes:
  1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Clone No.: B5J5
• Immunogen: Mouse CD137 human Fc fusion protein
• Target: TNFRSF9
• Purification: The antibody was purified by affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.3, and 0.02% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD137; ILA; Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA; CD137

Quick Database Links

Gene Symbol

TNFRSF9

UniProt

P20334

NCBI Gene ID

21942