CD131 (Phospho-Y593) Antibody

• Catalog Number: orb216075
• Category: Antibodies
• Description: The CD131 (Phospho-Y593) Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y593 of human CD131 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Sheep
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y593 of human CD131 protein. The exact sequence is proprietary.
• UniProt ID: P32927
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL3RB; IL5RB; Cytokine receptor common subunit bet
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 1439

Western blot analysis of CD131 (Phospho-Y593) expression in MCF7 IL1b-treated (A), HepG2 LPS-treated (B) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 130 kD)

Immunohistochemical analysis of CD131 (Phospho-Y593) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.