Anti-CD131 [BION-1]

• Catalog Number: orb669740
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD131
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BION-1
• Tested applications: Blocking, Functional Studies, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD131
• UniProt ID: P32927
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CSF2RB; CD131; IL3RB; IL-3R common beta subunit; I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-CD131 antibody BION-1. U937 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of BION-1 ((orb669741, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of U937 cells using anti-CD131 antibody BION-1. Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of BION-1 (orb669741) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb669741, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.