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Anti-CD129 (Phospho-S519) Antibody

Catalog Number: orb1474757

DispatchUsually dispatched within 5-10 working days
$ 190.00
Catalog Numberorb1474757
CategoryAntibodies
DescriptionRabbit polyclonal antibody to CD129 (phospho-S519)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S519 of human CD129 protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetIL9R
Entrez3581
UniProt IDQ01113
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesInterleukin-9 receptor; IL-9 receptor; IL-9R; CD12
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-CD129 (Phospho-S519) Antibody

Western blot analysis of CD129 (Phospho-S519) expression in HuT78 (A) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 57 kD)

Anti-CD129 (Phospho-S519) Antibody

Immunohistochemical analysis of CD129 (Phospho-S519) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-CD129 (Phospho-S519) Antibody

Immunofluorescent analysis of CD129 (Phospho-S519) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.