Cart summary

You have no items in your shopping cart.

Anti-CD129 (Phospho-S519) Antibody

Catalog Number: orb1474757

DispatchUsually dispatched within 5-10 working days
$ 190.00
Catalog Numberorb1474757
CategoryAntibodies
DescriptionRabbit polyclonal antibody to CD129 (phospho-S519)
TargetIL9R
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S519 of human CD129 protein. The exact sequence is proprietary.
UniProt IDQ01113
Tested applicationsIF, IH, WB
Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesInterleukin-9 receptor; IL-9 receptor; IL-9R; CD12
Read more...
NoteFor research use only
Entrez3581
Anti-CD129 (Phospho-S519) Antibody

Western blot analysis of CD129 (Phospho-S519) expression in HuT78 (A) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 57 kD)

Anti-CD129 (Phospho-S519) Antibody

Immunohistochemical analysis of CD129 (Phospho-S519) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-CD129 (Phospho-S519) Antibody

Immunofluorescent analysis of CD129 (Phospho-S519) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.