Anti-CD11a [MHM24]

• Catalog Number: orb669761
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD11a
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MHM24
• Tested applications: FA, FC
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: Spleen cells from BALB/c mice immunised with EBV-transformed human lymphoblastoid cells were fused with P3-NSUlAg4.1 myeloma cells and the resulting hybridomas were screened for antibody production by a radioimmunoassay on T and B cell lines.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD11a
• UniProt ID: P20701
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Integrin alpha-L; CD11 antigen-like family member
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD11a antibody MHM24. Paraformaldehyde fixed Jurkat cells were stained with anti-unknown specificity antibody (orb256449; isotype control, black line) or the mouse IgG1 version of MHM24 (orb669760, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Jurkat cells using anti-CD11a MHM24. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells stained with the chimeric mouse IgG version of MHM24 at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb669758, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (orb256449) followed by Alexa Fluor® 488 secondary antibody.

Immunofluorescence staining of Jurkat cells using anti-CD11a antibody MHM24. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of MHM24 (orb669761) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing diffuse membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb669761, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.