Anti-CD106 [B-K9 (C106-K9)]

• Catalog Number: orb758940
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD106
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: B-K9 (C106-K9)
• Tested applications: Blocking, FC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: This antibody was raised by immunizing Balb/c mice against activated human umbilical vein endothelial cells (HUVEC).
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD106
• UniProt ID: P19320
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: VCAM1; Vascular cell adhesion protein 1; V-CAM 1;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD106 antibody B-K9 (C106-K9). Paraformaldehyde fixed K562 cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of B-K9 (C106-K9) (orb758941, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-CD106 antibody B-K9 (C106-K9). Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of B-K9 (C106-K9) (orb758941) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758941, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.