Anti-CD10 [NL-1]

SKU: orb613956

Description

Mouse monoclonal antibody to CD10

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IP, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: NL-1
• Immunogen: BALB/c mice were immunised with acute lymphocytic leukaemia cells from a human leukaemia patient. Splenocytes were obtained from immunised mice and fused with MOPC-21 NS/1 myeloma cells to generate a hybridoma.
• Target: CD10
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Neprilysin; Cluster of Differentiation 10; Membrane metallo-endopeptidase; MME; Neutral endopeptidase 24.11; Neutral endopeptidase; NEP; Common acute lymphocytic leukaemia antigen; CALLA; Skin fibroblast elastase; SFE; CMT2T; SCA43; Atriopeptidase; Enkephalinase

Immunofluorescence staining of fixed Jurkat cells with anti-CD105 antibody NL-1. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of NL-1 (orb613957) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing punctate cytoplasmic staining, likely from protein trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613957, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD10

UniProt

P08473