Anti-CCRL2 [BZ5B8]

• Catalog Number: orb758804
• Category: Antibodies
• Description: Rat monoclonal antibody to CCRL2
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: BZ5B8
• Tested applications: ELISA, FA, FC, ICC, IHC, IP, WB
• Reactivity: Mouse
• Isotype: IgG2a
• Immunogen: This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1–24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CCRL2
• UniProt ID: O35457
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CCR11; C-C chemokine receptor-like 2; Chemokine re
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the anti-CCRL2 antibody BZ5B8. RAW264.7 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of BZ5B8 (orb758803, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-CCRL2 antibody BZ5B8 (B467). Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of BZ5B8 (orb758803) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758803, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CCRL2 antibody Mouse thymus (A) and mouse brain (B) tissue lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of CCRL2 (orb758803) at 0.5 µg/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.