Anti-CCR5 [T21/8]

SKU: orb758950

Description

Mouse monoclonal antibody to CCR5

Images & Validation

• Tested Applications: ELISA, FC, IF, IP, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: T21/8
• Immunogen: This antibody was raised by immunizing mice with intact RBL-CCR5 cells.
• Target: CCR5
• Purity: Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

CD195; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CHEMR13; HIV-1 fusion coreceptor

Flow cytometry using the Anti-CCR5 antibody T21/8. Paraformaldehyde fixed U937 cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of T21/8 (orb758951, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed U937 cells with anti-CCR5 antibody T21/8. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version T21/8 (orb758951) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758951, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CCR5

UniProt

P51681