Anti-CCR5 [T21/8]

• Catalog Number: orb758950
• Category: Antibodies
• Description: Mouse monoclonal antibody to CCR5
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: T21/8
• Tested applications: ELISA, FC, IF, IP, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: This antibody was raised by immunizing mice with intact RBL-CCR5 cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CCR5
• UniProt ID: P51681
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD195; C-C chemokine receptor type 5; C-C CKR-5; C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CCR5 antibody T21/8. Paraformaldehyde fixed U937 cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of T21/8 (orb758951, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed U937 cells with anti-CCR5 antibody T21/8. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version T21/8 (orb758951) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758951, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.