Anti-CCR5 [ST6]

• Catalog Number: orb1463370
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CCR5
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: ST6
• Tested applications: Depletion, ELISA, FC, SBR
• Reactivity: Human, Monkey
• Isotype: Rabbit IgG1
• Immunogen: This antibody was isolated from a phage displayed antibody fragment library. The library was generated from a New Zealand White rabbit immunised with purified CCR5-N-GST protein, and four rounds of panning were completed against immobilised CCR5-N-MBP antigen.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CCR5
• UniProt ID: P51681
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD195; C-C chemokine receptor type 5; C-C CKR-5; C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CCR5 antibody ST6. Paraformaldehyde fixed U937 cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of ST6 (orb1463369, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed U937 cells with anti-CCR5 antibody ST6. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of ST6 (orb1463369) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1463369, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CCR5 antibody ST6. K562 cell lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of ST6 (orb1463369) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.