Anti-CCR5 (phosphoserine 337) [V14/2]

• Catalog Number: orb758954
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CCR5 (phosphoserine 337)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: V14/2
• Tested applications: ELISA, FC, IF, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: This antibody was raised by immunizing mice with a synthetic phospho peptide corresponding to the C-Terminal region of human CCR5 conjugated to BSA.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CCR5 (phosphoserine 337)
• UniProt ID: P51681
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD195; C-C chemokine receptor type 5; C-C CKR-5; C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the anti-CCR5 (phosphoserine 349) antibody E11/19. U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of E11/19 (orb758954, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed U937 cells with anti-CCR5 (phosphoserine 337) antibody V14/2. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of V14/2 (orb758954) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758954, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CCR5 (phosphoserine 337) antibody V14/2 K562 cell lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of V14/2 (orb758954) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.