Anti-CCR1 antibody(DMC465); IgG1 Chimeric mAb

• Catalog Number: orb1743101
• Category: Antibodies
• Description: Anti-CCR1 antibody(DMC465); IgG1 Chimeric mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DMC465
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Rabbit/Human Fc chimeric IgG1
• Antibody Type: Recombinant Antibody
• Dilution range: Flow Cyt 1:100; WB 1:1000
• Conjugation: Unconjugated
• Target: CCR1
• UniProt ID: P32246
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD191; CKR-1; CKR1; CMKBR1; HM145; MIP1aR; SCYAR1
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

CCR1 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CCR1 on Expi293 cells transfected with human CCR1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Anti-CCR1 antibody (SKU# orb1743101) at 1/1000 dilution.Lane: Hela, whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 41 kDa.Observed band size: 50 kDa

Flow cytometry analysis of antigen binding of anti-human CCR1 mAb (orb1743101). (A) orb1743101 does not bind to CHO-S cells that do not express CCR1. (B) A clear peak shift of orb1743101 was seen compared to the control when incubated with CCR1-expressing 8226 cells, indicating strong binding of orb1743101 to CCR1. Antibodies were incubated at 5 μg/ml.