CCDC47 Rabbit Polyclonal Antibody

SKU: orb666205

Description

Rabbit polyclonal antibody to CCDC47

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human CCDC47. The exact sequence is proprietary.
• Target: CCDC47
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Coiled-coil domain-containing protein 47 antibody

Western blot analysis of CCDC47 expression in LO2 (A), mouse lung (B) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 60 kD)

Immunofluorescent analysis of CCDC47 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

CCDC47

UniProt

Q96A33, Q9D024, Q5U2X6

NCBI Gene ID

303606, 67163, 57003