CBX8 Antibody

• Catalog Number: orb1473665
• Category: Antibodies
• Description: The CBX8 Antibody is suitable for FC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CBX8. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: This antibody is purified through a protein G column.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CBX8. The exact sequence is proprietary.
• UniProt ID: Q9HC52
• Tested applications: FC, WB
• Dilution range: WB (1/500 - 1/1000), FC (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PC3; RC1; Chromobox protein homolog 8; Polycomb 3
• Note: For research use only
• Entrez: 57332

Western blot analysis of CBX8 expression in 293 (A), Hela (B), A549 (C) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43 kD)

Flow cytometric analysis of A549 cells using Anti-CBX8 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.