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Anti-Catalase (Phospho-Y386) Antibody

Catalog Number: orb216045

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb216045
CategoryAntibodies
DescriptionRabbit polyclonal antibody to Catalase (Phospho-Y386)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIH, WB
ReactivityHuman, Mouse, Rat, Zebrafish
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y386 of human Catalase protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1:1000, IHC-P: 1:100-1-200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetCAT
Entrez24248, 847, 12359
UniProt IDP04040, P04762, P24270
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti Catalase antibody
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Catalase (Phospho-Y386) Antibody

Western blot analysis of Catalase (Phospho-Y386) expression in AML12 (A) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 60 kD)

Anti-Catalase (Phospho-Y386) Antibody

Immunohistochemical analysis of Catalase (Phospho-Y386) staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Catalase (Phospho-Y386) Antibody

Direct ELISA antibody dose-response curve using Anti-Catalase (Phospho-Y386) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.