Caspase 9 Mouse Monoclonal Antibody

SKU: orb323116

Description

The Caspase 9 Antibody is suitable for IF, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence of human Caspase 9. The exact sequence is proprietary. Purification: Affinity chromatography

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IF, IP, WB
• Dilution Range: WB: 1:1000-1:3000, IP: 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Caspase 9. The exact sequence is proprietary.
• Target: CASP9
• Purification: Affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6

Western blot analysis of Caspase 9 expression in Hela (A), Jurkat (B), HepG2 (C), mouse heart (D), rat heart (E) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 47 kD)

Immunofluorescent analysis of Caspase 9 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation of Caspase 9 from 0.5 mg Hela whole cell extract lysate, using Anti-Caspase 9 Antibody.

Quick Database Links

Gene Symbol

CASP9

UniProt

P55211

NCBI Gene ID

842