Mouse Monoclonal Antibody to Cardiac Troponin I

SKU: orb613846

Description

Anti-Cardiac Troponin I [scFv 180], Mouse IgG1, Lambda

Research Area

Cell Biology, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: WB
• Reactivity: Human

Key Properties

• Host: Gallus
• Clonality: Monoclonal
• Isotype: Gallus IgG1
• Clone No.: scFv 180
• Immunogen: Cardiac Troponin I peptide (KISASRKLQLKT).
• Target: Cardiac Troponin I
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TNNI3; cTnI; cTn-I

Immunofluorescence staining of fixed HeLa cells with anti-Cardiac Troponin I antibody scFv 180. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of scFv 180 (orb613846) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613846, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-Cardiac Troponin I antibody scFv 180 Human heart lysate (35 µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of scFv 180 (orb613846) at 0.001 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for Cardiac Troponin I is 24.0 kDa. orb613846 successfully detected Cardiac Troponin I in human heart lysate.

Quick Database Links

Gene Symbol

Cardiac Troponin I

UniProt

P19429