Anti-CARD12 Antibody

• Catalog Number: orb412596
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NLRC4
• Target: NLRC4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant full length protein of human CARD12
• UniProt ID: F1M649, Q9NPP4, Q3UP24
• Tested applications: IF, WB
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-CARD12 antibody, anti-CLAN antibody, anti-CLA
• Note: For research use only
• Entrez: 58484, 268973, 298784

References

Zhang, Xuling et al. Effects of lysophosphatidic acid receptor 5 on NLRC4 inflammasome in brain tissues of transient cerebral ischemia/reperfusion rat Hum Exp Toxicol, 41, (2022)

PubMed: 35230166

Applications

WB

Reactivity

Rat

Western blot analysis of CARD12 expression in MCF7 (A), mouse heart (B), rat kidney (C) whole cell lysates. (Predicted band size: 116 kD; Observed band size: 110 kD)

Immunofluorescent analysis of CARD12 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.