Calcium Sensing Receptor (Phospho-T888) Antibody

• Catalog Number: orb213666
• Category: Antibodies
• Description: The Calcium Sensing Receptor (Phospho-T888) Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T888 of human Calcium Sensing Receptor protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Monkey, Mouse, Porcine, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T888 of human Calcium Sensing Receptor protein. The exact sequence is proprietary.
• UniProt ID: Q9QY96, P41180, P48442
• Tested applications: IF, WB
• Dilution range: WB: 1-500-1-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GPRC2A; PCAR1; Extracellular calcium-sensing recep
• Research Area: Epigenetics, GPCR, Neuroscience, Signaling Pathway
• Note: For research use only
• Entrez: 24247, 846, 12374

Western blot analysis of Calcium Sensing Receptor (Phospho-T888) expression in MCF7 (A), MDCK (B), U2OS (C), SP2/0 (D), PC12 (E), rat brain (F) whole cell lysates. (Predicted band size: 120 kD; Observed band size: 140 kD)

Immunofluorescent analysis of Calcium Sensing Receptor (Phospho-T888) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.