Anti-C5aR [S5/1]

• Catalog Number: orb758946
• Category: Antibodies
• Description: Mouse monoclonal antibody to C5aR
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: S5/1
• Tested applications: Blocking, ELISA, FC, Functional Studies, IP, WB
• Reactivity: Bovine, Ferret, Human, Rabbit
• Isotype: IgG2a
• Immunogen: This antibody was raised by immunizing mice with a synthetic peptide corresponding to C5a-R; amino acids: 1-31 (N terminal).
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: C5aR
• UniProt ID: P21730
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD88; C5a anaphylatoxin chemotactic receptor; C5a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed U937 cells with anti-C5aR antibody S5/1. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version S5/1 (orb758947) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758947, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.