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Item 1 of 2
Anti-c-Raf/B-RAF (Phospho-T491/599) Antibody
Catalog Number: orb315680
| Catalog Number | orb315680 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to c-Raf/B-RAF (Phospho-T491/599) |
| Target | RAF1; BRAF |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T491/599 of human c-Raf/B-RAF protein. The exact sequence is proprietary. |
| UniProt ID | P04049, Q99N57, P15056, P11345, P28028 |
| Tested applications | IH, WB |
| Dilution range | WB: 1-500:1000, IHC-P: 1-100:200 |
| Antibody Type | Primary Antibody |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti RAF proto-oncogene serine/threonine-protein k Read more... |
| Note | For research use only |
| Entrez | 109880, 24703, 5894, 110157, 673 |
Western blot analysis of c-Raf/B-RAF (Phospho-T491/599) expression in HEK293T (A), HeLa (B) whole cell lysates. (Predicted band size: 73; 84 kD; Observed band size: 84 kD)
Immunohistochemical analysis of c-Raf/B-RAF (Phospho-T491/599) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
