Anti-c-Jun Antibody

• Catalog Number: orb214141
• Category: Antibodies
• Description: Rabbit polyclonal antibody to JUN
• Target: JUN
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-Jun. The exact sequence is proprietary.
• UniProt ID: P05412, P05627, P17325
• Tested applications: ChIP, FC, IH, IP, WB
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100, FACS: 1-100-1-300
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Transcription factor AP-1 antibody, anti Acti
• Note: For research use only
• Entrez: 24516, 3725, 16476

Western blot analysis of c-Jun expression in PC3 (A), H1688 (B) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 43 kD)

Immunohistochemical analysis of c-Jun staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunoprecipitation of c-Jun from 0.5 mg HEK293T whole cell extract lysate, using 5 ug of Anti-c-Jun Antibody and 50 ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10 min, HEK293T whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 min under agitation. Proteins were eluted by addition of 40 ul SDS loading buffer and incubated for 10 min at 70°C; 10 ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with Anti-c-Jun Antibody.

ChIP analysis of Human endothelial cells (EA.hy926), incubated for 10 hours at 4°C. Cross-linking (X-ChIP) using formaldehyde for 10 minutes. Positive control: Position 89340150-89340297 in chromosome 11 (has a validated c-Jun site). Negative Control: Igr5 intron 3 (contains no c-Jun binding site). Detection step: Real-time PCR.