Anti-BTN3A1 antibody(DM94); Rabbit mAb

• Catalog Number: orb1174074
• Category: Antibodies
• Description: Anti-BTN3A1 antibody(DM94), Rabbit mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DM94
• Tested applications: ELISA, FC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Recombinant Antibody
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: BTN3A1
• UniProt ID: O00481
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BTN3A1, BTF5, CD277, BTN3.1, BT3.1
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human BTN3A1 protein, mFc-His tagged protein orb689408 can bind Rabbit anti-BTN3A1 monoclonal antibody (clone: DM94) in a linear range of 0.64-80 ng/ml.

Flow cytometry analysis with Anti-BTN3A1 on Expi293 cells transfected with human BTN3A1 (Red histogram) or Expi293 transfected with irrelevant protein (Blue histogram).

Flow cytometry analysis of antigen binding of rabbit anti-human BTN3A1 mAb (orb1174074). (A) orb1174074 does not bind to MCF-7 cells that do not express BTN3A1. (B) A clear peak shift of orb1174074 was seen compared to the control when incubated with BTN3A1-expressing 8226 cells, indicating strong binding of orb1174074 to BTN3A1. Antibodies were incubated at 2 μg/mL.

Anti-BTN3A1 antibody (SKU# orb1174074) at 1/1000 dilution.Lane: RPMI8226, whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 33 kDa.Observed band size: 35, 54, 68 kDa