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Item 1 of 3
Anti-BTK (Phospho-Y223) Antibody
Catalog Number: orb216039
| Catalog Number | orb216039 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to BTK (Phospho-Y223) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse, Rat |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y223 of human BTK protein. The exact sequence is proprietary. |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:500-1:1000 |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Target | BTK |
| Entrez | 695, 12229 |
| UniProt ID | P35991, Q06187 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Alternative names | anti AGMX1 antibody, anti ATK antibody, anti BPK a Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of BTK (Phospho-Y223) expression in mouse spleen (A), mouse liver (B) whole cell lysates. (Predicted band size: 76 kD; Observed band size: 75 kD)
Immunofluorescent analysis of BTK (Phospho-Y223) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Direct ELISA antibody dose-response curve using Anti-BTK (Phospho-Y223) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
