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Anti-BRSK1 Antibody

Catalog Number: orb1474731

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb1474731
CategoryAntibodies
DescriptionRabbit polyclonal antibody to BRSK1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human BRSK1. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetBRSK1
Entrez381979, 84446
UniProt IDQ8TDC3, Q5RJI5
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesKIAA1811; SAD1; SADB; Serine/threonine-protein kin
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-BRSK1 Antibody

Western blot analysis of BRSK1 expression in Hela (A), CT26 (B) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 87 kD)

Anti-BRSK1 Antibody

Immunofluorescent analysis of BRSK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    ELISA,  FC,  ICC,  IF,  IHC,  WB

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