Anti-BRAF V600E protein [600E-7]

• Catalog Number: orb1821607
• Category: Antibodies
• Description: Anti-BRAF V600E protein [600E-7]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 600E-7
• Tested applications: ELISA
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: The original antibody was generated by immunizing mice with double repeat recombinant BRAF V600E protein 'GDFGLATEKSRW' and BRAF V600 protein 'GDFGLATVKSRW' in equal amount of complete Freund’s adjuvant.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: BRAF V600E protein
• UniProt ID: P15056
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: RAFB1; BRAF1; p94; BRAF V600E kinase; Serine/threo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of with anti-BRAF V600E protein 600E-7. Immunofluorescence analysis of permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of 600E-7 (orb1821607) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1821607, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.