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Anti-BIM (Phospho-S69) Antibody

Catalog Number: orb235046

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb235046
CategoryAntibodies
DescriptionRabbit polyclonal antibody to BIM (phospho-S69)
TargetBCL2L11
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat, Sheep
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S69 of human BIM protein. The exact sequence is proprietary.
UniProt IDO54918, O88498, O43521
Tested applicationsIF, IH, WB
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesanti BIM antibody
NoteFor research use only
Entrez10018, 12125, 64547
Anti-BIM (Phospho-S69) Antibody

Western blot analysis of BIM (Phospho-S69) expression in HEK293T (A), Raw264.7 (B) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 25 kD)

Anti-BIM (Phospho-S69) Antibody

Immunohistochemical analysis of BIM (Phospho-S69) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-BIM (Phospho-S69) Antibody

Immunofluorescent analysis of BIM (Phospho-S69) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).