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Anti-BIK (Phospho-S35) Antibody

Catalog Number: orb515522

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb515522
CategoryAntibodies
DescriptionRabbit polyclonal antibody to BIK (phospho-S35)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S35 of human BIK protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1000, IHC-P: 1:50-100
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetBIK
Entrez638, 12124
UniProt IDQ13323, O70337
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesNBK; Bcl-2-interacting killer; Apoptosis inducer N
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-BIK (Phospho-S35) Antibody

Western blot analysis of BIK (Phospho-S35) expression in mouse embryo (A), rat ovary (B), HEK293T-EGF (C), HEK293T (D) whole cell lysates. (Predicted band size: 18 kD; Observed band size: 18 kD)

Anti-BIK (Phospho-S35) Antibody

Immunohistochemical analysis of BIK (Phospho-S35) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-BIK (Phospho-S35) Antibody

Direct ELISA antibody dose-response curve using Anti-BIK (Phospho-S35) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.