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Anti-BIK (Phospho-S35) Antibody

Catalog Number: orb515522

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb515522
CategoryAntibodies
DescriptionRabbit polyclonal antibody to BIK (phospho-S35)
TargetBIK
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S35 of human BIK protein. The exact sequence is proprietary.
UniProt IDQ13323, O70337
Tested applicationsIH, WB
Dilution rangeWB: 1:500-1000, IHC-P: 1:50-100
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesNBK; Bcl-2-interacting killer; Apoptosis inducer N
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NoteFor research use only
Entrez638, 12124
Anti-BIK (Phospho-S35) Antibody

Western blot analysis of BIK (Phospho-S35) expression in mouse embryo (A), rat ovary (B), HEK293T-EGF (C), HEK293T (D) whole cell lysates. (Predicted band size: 18 kD; Observed band size: 18 kD)

Anti-BIK (Phospho-S35) Antibody

Immunohistochemical analysis of BIK (Phospho-S35) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-BIK (Phospho-S35) Antibody

Direct ELISA antibody dose-response curve using Anti-BIK (Phospho-S35) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.