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Anti-Beta-glucosidase 2 Antibody

Catalog Number: orb214447

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb214447
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PRKCSH
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human Beta-glucosidase 2. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IF/ICC: 1-100-1-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetPRKCSH
Entrez5589, 19089
UniProt IDO08795, P14314
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti G19P1 antibody, anti Glucosidase 2 subunit be
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Beta-glucosidase 2 Antibody

Western blot analysis of Beta-glucosidase 2 expression in A375 (A), MCF7 (B), mouse brain (C) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 87 kD)

Anti-Beta-glucosidase 2 Antibody

Immunohistochemical analysis of Beta-glucosidase 2 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Beta-glucosidase 2 Antibody

Immunofluorescent analysis of Beta-glucosidase 2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Anti-Beta-glucosidase 2 Antibody [orb666750]

    WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 50 μl
  • PRKCSH peptide [orb217803]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg