Anti-Beta-2 microglobulin [BBM.1]

• Catalog Number: orb613793
• Category: Antibodies
• Description: Rabbit monoclonal antibody to beta2 microglobulin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BBM.1
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: Molt 4, a human T cell line.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Beta-2 microglobulin
• UniProt ID: P61769
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: B2M; Beta-2-microglobulin; CDABP0092; HDCMA22P; BB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-Beta-2 microglobulin antibody BBM.1 HeLa cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of BBM.1 (orb613793, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunofluoresence staining of fixed HeLa cells with anti-Beta-2 microglobulin antibody BBM.1. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of BBM.1 (orb613793) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613793, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-Beta-2 microglobulin antibody BBM.1 HeLa cell lysate (35 µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 (orb613793) at 0.03 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. orb613793 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.