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Anti-Beta-1 Adrenergic Receptor Antibody

Catalog Number: orb318975

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb318975
CategoryAntibodies
DescriptionRabbit polyclonal antibody to beta 1 Adrenergic Receptor
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human Beta-1 Adrenergic Receptor. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1:1000, IHC-P: 1:100-1:200, IF/ICC: 1:100-1:500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetADRB1
Entrez153
UniProt IDP08588
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti-ADR B1 antibody, anti-ADRB 1 antibody, anti-A
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Beta-1 Adrenergic Receptor Antibody

Western blot analysis of Beta-1 Adrenergic Receptor expression in mouse heart (A), rat heart (B), rat kidney (C) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 51 kD)

Anti-Beta-1 Adrenergic Receptor Antibody

Immunohistochemical analysis of Beta-1 Adrenergic Receptor staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Beta-1 Adrenergic Receptor Antibody

Immunofluorescent analysis of Beta-1 Adrenergic Receptor staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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