Anti-BCKDHB Antibody

• Catalog Number: orb1880935
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BCKDHB
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human BCKDHB. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 594
• UniProt ID: P21953
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Alternative names: 2-oxoisovalerate dehydrogenase subunit beta, mitoc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BCKDHB expression in Hela (A), Jurkat (B), Daudi (C) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 35 kD)

Immunofluorescent analysis of Anti-BCKDHB staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).