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Item 1 of 2
Anti-BCKDHB Antibody
Catalog Number: orb1880935
| Catalog Number | orb1880935 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to BCKDHB |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Reactivity | Human |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human BCKDHB. The exact sequence is proprietary. |
| Antibody Type | Primary Antibody |
| Dilution range | WB (1/500 - 1/1000), IF/IC (1/10 - 1/50) |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Entrez | 594 |
| UniProt ID | P21953 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | 2-oxoisovalerate dehydrogenase subunit beta, mitoc Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of BCKDHB expression in Hela (A), Jurkat (B), Daudi (C) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 35 kD)
Immunofluorescent analysis of Anti-BCKDHB staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
