Anti-BAFF (belimumab biosimilar) mAb

• Catalog Number: orb1173655
• Category: Antibodies
• Description: Anti-BAFF (belimumab biosimilar) mAb
• Species/Host: Human
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Antibody Type: Biosimilar Antibody
• Dilution range: ELISA 1:5000-10000; Flow Cyt 1:100
• Form/Appearance: Powder
• Conjugation: Unconjugated
• Target: BAFF
• UniProt ID: Q9Y275
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TNFSF13B;BAFF;BLYS;CD257;DTL;TALL1;THANK;TNFSF20;Z
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human BAFF, hFc tagged protein (orb689402) can bind Anti-BAFF Neutralizing antibody (orb1173655) in a linear range of 3.2-400 ng/ml. In order to specifically detect orb1173655, mouse anti-human Fab-specific antibody was used as detection antibody.

Flow cytometry analysis with Anti-BAFF mAb 1 μg/ml (belimumab) on Expi293 cells transfected with human BAFF (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).