Anti-BACE2 [1/ 9 ]

• Catalog Number: orb613859
• Category: Antibodies
• Description: Mouse monoclonal antibody to BACE2
• Target: BACE2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: Human BACE2 244–333.
• UniProt ID: Q9Y5Z0
• Tested applications: IHC, WB
• Clone Number: 1/ 9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Beta-secretase 2; Aspartic-like protease; Aspartyl
• Note: For research use only

Immunofluorescence staining of fixed U251 cells with anti-BACE2 antibody 1/9. Immunofluorescence analysis of paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 1/9 (orb613859) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613859, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-BACE2 antibody 1/9 HeLa (A) and U251 (B) cell lysate samples (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of 1/9 (orb613859) at 1 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for BACE2 is 56.2 kDa. orb613859 successfully detected BACE2 in HeLa and U251 cell lysate samples.