B9D1 Rabbit Polyclonal Antibody

SKU: orb341408

Description

Rabbit polyclonal antibody to B9D1

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:500-1:2000, IHC-P: 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human B9D1
• Target: B9D1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MKSR1; B9 domain-containing protein 1; MKS1-related protein 1

Western blot analysis of B9D1 expression in Hela (A), mouse lung (B), mouse kidney (C) whole cell lysates. (Predicted band size: 16; 22 kD; Observed band size: 23 kD)

Immunohistochemical analysis of B9D1 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of B9D1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

B9D1

UniProt

Q9R1S0, Q9UPM9, P0C5J2

NCBI Gene ID

287383, 27078, 100911746, 27077