Anti-B7-H6 [17B1.3]

• Catalog Number: orb758944
• Category: Antibodies
• Description: Mouse monoclonal antibody to B7-H6
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 17B1.3
• Tested applications: FC, IF
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Immunised mice with extracellular domain of human B7-H6 and selected on the basis of specific reactivity by P815.B7-H6, but not with P815.B7-H1.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: B7-H6
• UniProt ID: Q68D85
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: B7H6; B7 homolog 6; Natural cytotoxicity triggerin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-B7-H6 antibody 17B1.3. Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 17B1.3 (orb758945, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed K562 cells with anti-B7-H6 antibody 17B1.3. Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 17B1.3 (orb758945) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758945, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.