Anti-B7-H5(onvatilimab biosimilar) mAb

• Catalog Number: orb1291214
• Category: Antibodies
• Description: Anti-B7-H5(onvatilimab biosimilar) mAb
• Species/Host: Human
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Antibody Type: Biosimilar Antibody
• Dilution range: ELISA 1:5000-10000|Flow Cyt 1:100
• Form/Appearance: Powder
• Conjugation: Unconjugated
• Target: B7-H5
• UniProt ID: Q9H7M9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: VISTA;VSIR;Sisp-1;C10orf54;DD1alpha;Dies1;GI24;PD-
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human B7-H5 Protein, hFc Tag (orb1173726) can bind Anti-B7-H5 Neutralizing antibody (orb1291214) in a linear range of 2.44–625.00 ng/ml. In order to specifically detect orb1291214, mouse anti-human Fab-specific antibody was used as detection antibody.

Flow cytometry analysis with 1 μg/mL Anti-B7-H5 (onvatilimab biosimilar) mAb (orb1291214) on Expi293 cells transfected with Human B7-H5 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Flow cytometry analysis of antigen binding of anti-human B7-H5 mAb (orb1291214). (A) orb1291214 does not bind to 293T cells that do not express B7-H5. (B) A clear peak shift of orb1291214 was seen compared to the control when incubated with B7-H5-expressing THP-1 cells, indicating strong binding of orb1291214 to B7-H5. Antibodies were incubated at 5 μg/mL.