B7-H2 Antibody

• Catalog Number: orb1174069
• Category: Antibodies
• Description: Anti-B7-H2 antibody(DM99), Rabbit mAb
• Target: B7-H2
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: O75144
• Tested applications: ELISA, FC
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DM99
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, I
• Note: For research use only

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-H2 protein, mFc-His tagged protein orb689390 can bind Rabbit anti-B7-H2 monoclonal antibody (clone: DM99) in a linear range of 3.2-80 ng/ml.

Flow cytometry analysis with Anti-B7-H2 (DM99) on Expi293 cells transfected with human B7-H2 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Flow cytometry analysis of antigen binding of rabbit anti-human B7-H2 mAb (orb1174069). (A) orb1174069 does not bind to Jurkat cells that do not express B7-H2. (B) A clear peak shift of orb1174069 was seen compared to the control when incubated with B7-H2-expressing Siha cells, indicating strong binding of orb1174069 to B7-H2. Antibodies were incubated at 5 μg/mL.