Anti-ATPIF1 Antibody

• Catalog Number: orb412324
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATPIF1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant full length protein of human ATPIF1
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ATPIF1
• Entrez: 93974, 11983
• UniProt ID: O35143, Q9UII2
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-ATPI antibody, anti-ATPase inhibitor, mitocho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATPIF1 expression in MCF7 (A), Hela (B), mouse brain (C) whole cell lysates. (Predicted band size: 6; 7; 12 kD; Observed band size: 12 kD)

Immunofluorescent analysis of ATPIF1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).