ATP5J Antibody

SKU: orb666100

Description

Rabbit polyclonal antibody to ATP5J

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human ATP5J. The exact sequence is proprietary.
• Target: ATP5J
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Anti-ATP5A antibody, anti-ATPM antibody, anti-ATP synthase-coupling factor 6 mitochondrial antibody, anti-ATPase subunit F6 antibody

Western blot analysis of ATP5J expression in THP1 (A), mouse kidney (B) whole cell lysates. (Predicted band size: 12; 13 kD; Observed band size: 13 kD)

Immunohistochemical analysis of ATP5J staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of ATP5J staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

ATP5J

UniProt

P18859, P97450, P21571

NCBI Gene ID

94271, 11957, 522