Anti-ATG5 Antibody

• Catalog Number: orb1474566
• Category: Antibodies
• Description: Rabbit monoclonal antibody to ATG5
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic peptide of human APG5L/ATG5
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: ATG5
• Entrez: 9474, 11793
• UniProt ID: Q3MQ06, Q99J83, Q9H1Y0
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: APG5L; ASP; Autophagy protein 5; APG5-like; Apopto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATG5 expression in Raw264.7 (A), K562 (B), C6 (C), Hela (D) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 55 kD)

Immunofluorescent analysis of ATG5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.