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Anti-ATG14 Antibody

Catalog Number: orb412630

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb412630
CategoryAntibodies
DescriptionRabbit polyclonal antibody to ATG14
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human ATG14
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-2000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetATG14
Entrez22863, 100504663, 305831
UniProt IDQ8CDJ3, Q6ZNE5, D4A4K3
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti-KIAA0831 antibody, anti-Beclin 1-associated a
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-ATG14 Antibody

Western blot analysis of ATG14 expression in Hela (A), mouse liver (B), rat brain (C), rat lung (D) whole cell lysates. (Predicted band size: 42; 55 kD; Observed band size: 60 kD)

Anti-ATG14 Antibody

Immunofluorescent analysis of ATG14 staining in BALB3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    ELISA,  IHC

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl
  • ATG14 Antibody [orb682409]

    ELISA,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • ATG14L Rabbit pAb [orb763862]

    IHC

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    50 μl, 100 μl