ATG14 Antibody

SKU: orb412630

Description

Rabbit polyclonal antibody to ATG14

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB: 1:500-2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human ATG14
• Target: ATG14
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

anti-KIAA0831 antibody, anti-Beclin 1-associated autophagy-related key regulator antibody, anti-Barkor antibody, anti-Autophagy-related protein 14-like protein antibody, anti-Atg14L antibody

Western blot analysis of ATG14 expression in Hela (A), mouse liver (B), rat brain (C), rat lung (D) whole cell lysates. (Predicted band size: 42; 55 kD; Observed band size: 60 kD)

Immunofluorescent analysis of ATG14 staining in BALB3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

ATG14

UniProt

D4A4K3, Q6ZNE5, Q8CDJ3

NCBI Gene ID

305831, 22863, 100504663