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Item 1 of 3
Anti-ATF5 Rabbit Monoclonal Antibody
Catalog Number: orb548102
| Catalog Number | orb548102 |
|---|---|
| Category | Antibodies |
| Description | Anti-ATF5 Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat. |
| Clonality | Monoclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Immunogen | A synthesized peptide derived from human ATF5 |
| UniProt ID | Q9Y2D1 |
| MW | 48 kDa |
| Tested applications | ICC, IF, IHC, IP, WB |
| Application notes | WB 1:1000-1:2000IHC 1:100-1:500ICC/IF 1:50-1:200IP 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL |
| Antibody Type | Primary Antibody |
| Clone Number | HDD-1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Cyclic AMP-dependent transcription factor ATF-5; c Read more... |
| Note | For research use only |
Western blot analysis of ATF5 expression in (1) Jurkat cell lysate; (2) 3T3 cell lysate; (2) C6 cell lysate.
Immunohistochemical analysis of paraffin-embedded Human breast, using ATF5 Antibody.
ICC staining of ATF5 in HeLa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS.
