Aquaporin 2 (Ser269) Antibody

SKU: orb319467

Description

Our Anti-Aquaporin 2 (Ser269) rabbit polyclonal phosphospecific primary antibody from Biorbyt is produced in-house. It detects human, mouse, and rat Aquaporin 2 (Ser269) and is antigen affinity purified from pooled serum.

Research Area

Kidney & Urological Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IHC, IP, WB
• Dilution Range: WB: 0.736111111, IHC: 1:600-1:5000, ICC: 1:200-1:500, IP: 3 ul
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser269 of rat aquaporin 2, conjugated to keyhole limpet hemocyanin (KLH).
• Target: Aquaporin 2 (Ser269)
• Molecular Weight: 29/37
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol.
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADH water channel antibody, AQP 2 antibody, AQP CD antibody, AQP-2 antibody, AQP-CD antibody, AQP2 antibody, AQP2_HUMAN antibody, AQPCD antibody, Aquaporin 2 collecting duct antibody, Aquaporin CD antibody, Aquaporin-2 antibody, Aquaporin-CD antibody, Aquaporin2 antibody, Aquaporine 2 antibody, Collecting duct water channel protein antibody, MGC34501 antibody, Water channel aquaporin 2 antibody, Water channel protein for renal collecting duct antibody, WCH CD antibody, WCH-CD antibody, WCHCD antibody

Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. orb319467, red, 1:1000) and total AQP2 (green). Nuclei labeled with DAPI. Magnification 600x.

Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. orb319467, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x.

Western analysis of rat kidney inner medullary (IM) lysate from ddAVP and ddAVP + ADM-treated IM probed for total (bottom) and pSer269 (Cat. orb319467, 1:1000)(top) AQP2. Brackets indicate the glycosylated AQP2 protein between 35 and 45 kDa, and the arrow indicates the un glycosylated AQP2 protein at 29 kDa. The matched pair comparisons were achieved by comparing Lane 1 with Lane 5; Lane 2 with Lane 6; Lane 3 with Lane 7; and Lane 4 with Lane 8.

Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser269 in the vasopressin (AVP) treated lane (+), but not in the control lane (-).

Quick Database Links

Gene Symbol

Aquaporin 2 (Ser269)

UniProt

P34080