Apolipoprotein L3 Rabbit Polyclonal Antibody

SKU: orb235020

Description

The Apolipoprotein L3 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Apolipoprotein L3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:500-1000, IHC-P: 1:100-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Apolipoprotein L3. The exact sequence is proprietary.
• Target: APOL3
• Molecular Weight: 44 kDa
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apolipoprotein L3; Apolipoprotein L-III; ApoL-III; TNF-inducible protein CG12-1; CG12_1

Immunofluorescent analysis of Apolipoprotein L3 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Immunohistochemical analysis of paraffin-embedded rat liver using Apolipoprotein L3 Antibody orb235020 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat lymph node using Apolipoprotein L3 Antibody orb235020 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat ovary using Apolipoprotein L3 Antibody orb235020 with dilution at 1:100.

Western blot analysis of mouse lung (1), rat testis (2), rat spleen (3) using Apolipoprotein L3 Antibody orb235020 with dilution at 1:1000.

Quick Database Links

Gene Symbol

APOL3

UniProt

O95236

NCBI Gene ID

80833